Composite

Part:BBa_K3584008:Design

Designed by: Kong Yangyang   Group: iGEM20_Shanghai_United   (2020-10-17)


p14 pro-rbs-tyrR-rbs-tyrP-PPO


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 798
    Illegal BglII site found at 1140
    Illegal BglII site found at 3083
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 604
    Illegal AgeI site found at 2035
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No


Source

The TyrR protein and tyrP gene comes from the genomic sequence of E. coli. The PPO comes from EC1.10.3.1.

References